Application

Research Sub CategoryHistones

This Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected Histone H3.3 in HeLa cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.3.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.